Study of Conjugation in E.Coli

STUDY OF CONJUGATION IN E.coli AIM : To study the meeting of bacterial conjugation through enthrall of factors,coding for antibiotic resistance. school of thought : From one bacterial nisus(donor) to a nonher strain(recipient) is a known crabby that occurs in nature with objective of mixing the element pool. In otherwise asexually reproducing organism, DNA polish off among bacteria is in different mediating ways: I. Transformation II. Transduction III. Conjugation Conjugation is the approximately widespread routine of transferring genetic material from one bacterial stallular phone to another. It is a process in which unidirectional transfer of DNA is arbitrate by conjugal plasmid or conjugal transposons requiring cell to cell contacts. It was discovered by Lederberg and Tatum. Conjugation is best soundless by considering properties of F-factor, which is a olive-sized circular plasmid DNA, that poop replicate autonomously in the cell or can shuffle int o host chromosome and thus transfer host chromosomal marker. The man-to-man cells with integrated F-factor ar stray and allowed to form pure colonies. The resulting straincan transfer chromosomal marker at very high frequency and termed as Hfr strain. INTRODUCTION: The E.coli strain A & B referred to as parental strains.

give A carries an antibiotic distasteful gene in F-plasmid coding for tetracycline, opus Strain B is detached of any F-factor, but carries streptomycin patient of gene in its chromosome. consequently both the strains grows on average containing tetracycline or streptomycin to which they are resistant on conjugating the both parental strains, the resulting cultur e go out be resistant to both the antibioti! cs, i.e. the conjugate sample when plate on a medium containing tetracycline or streptomycin willing survive suggesting that gene transfer has taken place from Strain A to B. thus far the parental strain when plated on the uniform medium does not survive as they are sore to either tetracycline or streptomycin. MATERIALS REQUIRED:...If you want to experience a encompassing essay, order it on our website: OrderEssay.net

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