Restriction Digest

labor Digest and Agarose Gel Electrophoresis of desoxyribonucleic acid use Restriction Sites in a Plasmid Pre-Lab: Before you buzz off to lab, you should read the ingredient in Campbell on confinement enzymes and agarose jelly electrophoresis (in chapter 21). In addition, you should introduce yourself to the following equipment casualty: plasmid, obstruction endonuclease, and agarose gel electrophoresis. These terms are discussed in Campbell, and can too be plant using various internet sources, e.g. http://www.accessexcellence.org/AE/AEC/CC/restriction.php In this exercise, you leave behind digest purified plasmid desoxyribonucleic acid with various restriction enzymes, either individually (single digest), or combine to work overher in pairs (double digests). You allow then separate the resulting fragments by agarose gel electrophoresis, and comparing the sizes of digested fragments with fragments of known size (DNA size standards). subsequently staini ng and photographing the gel, you entrust calculate the size of the specific fragments, and limit a alter restriction map based on your results. The plasmid you will digest is called pAMP. It is a circular double-stranded jot of DNA that contains a bacterial origin of replication (allowing it to double up in E. coli), and an ampicillin-resistance component (the product of this gene confers resistance to the antibiotic, ampicillin). office 1.

You will be given over a play of reagents, in individual microcentrifuge tobacco pipes. Because the labels tend to get stuck in the microcentrifuge, hit all labels and re-label each tube eyelid with a card shark pen. Make sure you read the confine o! f each tube before starting your experiment! quad of your tubes will already kick in restriction enzyme in them, and you will add reagents instantaneously to these reaction tubes. THE TUBES MUST STAY ON ICE!!! 2. strike out an empty microcentrifuge tube Uncut DNA. 3.You now have 5 reaction tubes, the one you fair(a) labeled, and the 4 others labeled with...If you penury to get a full phase of the moon essay, rank it on our website: OrderEssay.net

If you want to get a full information about our service, visit our page: write my essay